Aquatic environment

Optimization of process parameters using response surface methodology for bioconversion of activated sludge by Penicillium corylophilum


Sarkar Mannan , Ahmadun Fakhru’l-Razi , Md Zahangir Alam

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Received December 14, 2005,Revised July 04, 2006, Accepted , Available online

Volume 19,2007,Pages 23-28

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The quantitative interactions of process parameter on the activated sludge treatment in terms of biodegradation by Penicillium corylophilum was investigated using response surface methodology (RSM). The three parameters namely temperature of 33℃, agitation of 150 r/min and pH of 5 were chosen as center point from the previous study of fungal treatment. The experimental data of chemical oxygen demand (COD) removal (%) was fitted into a quadratic polynomial model using multiple regression analysis. The optimum process conditions were determined by analyzing response surface 3-dimentional surface plot and contour plot and also by solving the regression model equation with Design Expert software. Box-Behnken design technique under RSM were used to optimize their interactions, which showed that an incubation temperature of 32.5℃, agitation of 105 r/min and pH of 5.5 were the best conditions. Under these conditions the maximum predicted yield of COD removal was 98.43%. These optimum conditions were used to evaluate the trail experiment, and the maximum yield of COD removal was recorded as 98.5 %.

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