Environmental biology

Effects of cultivation conditions on the diversity of microbes involved in the conversion of rice straw to fodder

YANG Hong-yan , GAO Li-juan , WANG Xiao-fen , WANG Wei-dong , CUI Zong-jun


Received December 23, 2006,Revised April 11, 2006, Accepted , Available online

Volume 19,2007,Pages 67-73

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In order to confirm the suitable cultivation conditions for analyzing lactic acid bacteria communities and provide the cultivation foundation for lactic acid bacteria communities used to convert straw into fodder, fermented rice straw was inoculated into 13 broths. After 48 h cultivation, pH values, volatile products and microbial diversity were analyzed. Except for LAB broth, the pH values in the other broths could decrease to about 4.5. GC/MS analysis showed that lactic acid in Tomato MRS broth, MRS broth, LAB broth and Tomato juice broth was higher than that in the other broths. DNA concentration analysis showed that the counts of microbes in Tomato MRS broth were 2.5 times as much as those in other broths and suggested tomato juice could help reproduce the microbes. Denaturing gradient gel electrophoresis (DGGE) analysis showed that lactic acid bacteria species in HYA broth, Tomato juice broth and Tomato MRS broth were more than those in other broths.

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