Overexpression of NADH oxidase gene from Deinococcus geothermalis in Escherichia coli

Sase Kazuya , Iwasaki Tomomi , Karasaki Hatsune , Ishikawa Masahide


Received ,Revised , Accepted , Available online March 11, 2014

Volume ,2013,Pages S169-S171

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When using stable enzyme genes from a thermophile to create a biosensor in Escherichia coli, it is vital that these genes be overexpressed in order to provide a sufficient supply of enzymes. In this study, overexpression of the NADH oxidase (Nox) gene from the thermophile Deinococcus geothermalis was successfully achieved with the aim of creating a stable biosensor active at room temperatures. To do so, modification of 10 nucleotides, GAAATTAACT, upstream of the start codon of the Nox gene was necessary.

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