Environmental biology

Biodegradation of pyrene by Phanerochaete chrysosporium and enzyme activities in soils: Effect of SOM, sterilization and aging


Cuiping Wang , Hongwen Sun , Haibin Liu , Baolin Wang

DOI:10.1016/S1001-0742(13)60507-0

Received June 14, 2013,Revised September 17, 2013, Accepted , Available online April 01, 2014

Volume 26,2014,Pages 1135-1144

The impacts of soil organic matter (SOM), aging and sterilization on the production of lignin peroxidase (LiP) and manganese peroxidase (MnP) by Phanerochaete chrysosporium during the biodegradation of pyrene in soils were investigated. The biodegradation of pyrene by P. chrysosporium decreased with increasing SOM content, whereas the maximum activities of LiP and MnP increased, which indicates that SOM outweighed pyrene in controlling enzyme production. Sterilization enhanced the degradation of pyrene due to the elimination of competition from indigenous microbes, whereas aging led to a reduction in the degradation of pyrene primarily through changes in its sorbed forms. Both sterilization and aging could reduce SOM content and alter its structure, which also influenced the bioavailability of pyrene and the enzyme activity. The sterilization and aging processes caused changes in the degradation of pyrene, and the enzyme activities were greater in soils with high SOM contents. MnP was related to the degradation of pyrene to a greater extent, whereas LiP was more related to the decomposition of SOM.

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