Microbial response to CaCO3 application in an acid soil in southern China

Anning Guo , Longjun Ding , Zhong Tang , Zhongqiu Zhao , Guilan Duan


Received September 21, 2018,Revised , Accepted December 13, 2018, Available online December 24, 2018

Volume 31,2019,Pages 321-329

Calcium carbonate (CaCO3) application is widely used to ameliorate soil acidification. To counteract soil and bacterial community response to CaCO3 application in an acidic paddy soil in southern China, a field experiment was conducted with four different dosages of CaCO3 addition, 0, 2.25, 4.5 and 7.5 tons/ha, respectively. After one seasonal growth of rice, soil physicochemical properties, soil respiration and bacterial communities were investigated. Results showed that soil pH increased accordingly with increasing dose of CaCO3 addition, and 7.5 tons/ha addition increased soil pH to neutral condition. Moderate dose of CaCO3 application (4.5 tons/ha) significantly increased soil dissolved organic carbon (DOC) and dissolved organic nitrogen (DON) content, enhanced soil respiration, while the excessive CaCO3 application (7.5 tons/ha) decreased these soil properties. High-throughput sequencing results illustrated that moderate dose of CaCO3 application increased the richness and alpha diversity of soil bacterial community. Compared with control, the relative abundance of Anaerolineaceae family belonging to Chloroflexi phylum increased by 38.7%, 35.4% and 24.5% under 2.25, 4.5 and 7.5 tons/ha treatments, respectively. Redundancy analysis (RDA) showed that soil pH was the most important factor shaping soil bacterial community. The results of this study suggest that proper dose of CaCO3 additions to acid paddy soil in southern China could have positive effects on soil properties and bacterial community.

Copyright © Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. and Science Press. All rights reserved.京ICP备05002858号-3