TY - JOUR ID - TI - Effects of nicotinamide and riboflavin on the biodesulfurization activity of dibenzothiophene by Rhodococcus erythropolis USTB-03 AU - YAN Hai AU - SUN Xudong AU - XU Qianqian AU - MA Zhao AU - XIAO Chengbin and Ning Jun VL - 20 IS - 5 PB - SP - 613 EP - 618 PY - JF - Journal of Environmental Sciences JA - J. Environ. Sci. UR - http://www.jesc.ac.cn/jesc_en/ch/reader/view_abstract.aspx?file_no=2008200515&flag=1 KW - biodesulfurization; dibenzothiophene; nicotinamide; Rhodococcus erythropolis USTB-03; riboflavin AB - Rhodococcus erythropolis USTB-03 is a promising bacterial strain for the biodesulfurization of dibenzothiophene (DBT) via a sulfurspecific pathway in which DBT is converted to 2-hydroxybiphenyl (2HBP) as an end product. The effects of nicotinamide and riboflavin on the sulfur specific activity (SA) of DBT biodesulfurization by R. erythropolis USTB-03 were investigated. Both nicotinamide and riboflavin were found to enhance the expression of SA, which was not previously reported. When R. erythropolis USTB-03 was grown on a medium containing nicotinamide of 10.0 mmol or riboflavin of 50.0 mol, SA was raised from 68.0 or so to more than 130 mmol 2HBP/(kg dry cells h). When R. erythropolis USTB-03 was grown in the presence of both nicotinamide of 5.0 mmol and riboflavin of 25.0 mol, SA was further increased to 159.0 mmol 2HBP/(kg dry cells h). It is suggested that the biological synthesis of reduced form of flavin mononucleotide (FMNH2), an essential coenzyme for the activities of biodesulfurization enzyme Dsz C and A, might be enhanced by nicotinamide and riboflavin, which was responsible for the increased SA of R. erythropolis USTB-03. ER -