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The degradation of pesticide 1-(2-chlorobenzoyl)-3-(4-chlorophenyl)urea(CCU)in water and its accumulation and metabolism in fish were studied using HPLC method．The results of acute toxicity of both CCU and its naetabolites were also reported．The research demonstrated that CCU degraded quickly in aerobic aquatic environment and primary metabolites of CCU were 4-chlorophenyl urea and orthochlorobenzoic acid．Microorganisms play an important role for the degradation of CCU in water．Both the parents compound and its metabolites were not lethal to fish in tested concentration．The accumulation of CCU in fish was sunilar to that of dillubenzuron．The esterase in fish liver which could metabolized CCU was identified．The primary enzymatic degradation products of CCU is the same as that in water．
