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Received September 16, 1991,Revised , Accepted , Available online

Volume 4,1992,Pages 97-105

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With the super high sensitivity,high reproducibility and accumcy,nuclease P1 mediated 32P-postlabeling version has been successfully used to analyze the DNA-benzoquinone(DNA-BQ)adducts formed from m vitro cultured cells,reaction of benzoquinone ith calf thymus DNA and nucleoside monophosphates.It has been proven that the maior radioactive spot,contributing more than 70% of the total radioactivity of DNA-BQ adducts detected,is from deoxycytidine(dC)modified by benzoqumone while a minor one from deoxyguanosme(dGl.The method is capable of detecting 1 adduct in 109 to 1010 DNA bases.

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