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Two pollution zones in middle and lower reaches of Hanjiang River were selected for studying restoration processes. In each zones 6 stations were set up in upper stream of sewage outfall, 50m, 100 or 150m, 250 or 525m and 1250 or 3500m apart from the outfall. Chemical monitoring and microbial community biomonitoring were carried out simultaneously. Either the chemical monitoring or the biological monitoring proved the self-purification process of water body along with the increased distance from the sewage outfall. 4 biological parameters (species number of protozoa, percentage of phytomastigophora, diversity index and heterotrophy index) and parameter Seq of the colonization process all have statistically significant correlations with chemical comprehensive pollution indexes Pa and Pb.
