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In this paper, changes of enzymes involved in the degradation of recalcitrant aromatic pollutants from white rot fungus Phlebia radiata I-5-6 and cometabolism of biphenyl and varatryl alcohol by this fungus under nitrogen nutrition-rich were studied. Results from the experiment showed that C/N concentration ratio in the culture media played an important role on the activity of LiP. Under the condition of high concentration ratio of C/N or N/C, activity of LiP was higher, but higher activity of MnP only followed the low concentration of glucose or tatrate ammonium concentration in the media, when concentration of glucose or tatrate ammonium was more than 0.01 mol/L, the activity of MnP dropped down quickly. Veratryl alcohol increased the activity of LiP and MnP as well as the amount of .OH radical free under different concentration of nitrogen or carbon source; ascorbic acid, a scavenger of .OH radical free, brought the opposite effect to that of the veratryl alcohol on the LiP and MnP activity. Under nitrogen nutrition-rich condition removal percentage of biphenyl was lower, however, under cometabolic condition of veratryl alcohol and biphenyl, the degradation was enhanced obviously, moreover, intermediated products accumulated in the media during the cometabolic degradation process of biphenyl and veratryl alcohol was different from these which was found in the process of separate degradation of biphenyl or veratryl alcohol
