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The main effects of pollutions including acid rain, Cu2+, atrazine and their combined products on theactivities of urease, invertin, acid phosphatase and catalase were studied by means of orthogonal test. The resultsshowed that H+ and Cu2+ had significant influence on the activities of four enzymes and the ability of their inhibitingfollowed the order: H+ ＞ Cu2+ . Al3+ and atrazine only had litter effects on the activity of urease and phosphatase,respectively. Furthermore, interaction analysis revealed that Cu2+ -H+ affected on the activity of acid phosphatasesignificantly and antagonism on invertin and urease, Cu2+ -atrazine only exhibited the synergism on the activity ofacid phosphatase. But atrazine-H+ had non-interaction within the investigated concentration range. Among fourenzymes, acid phosphatase was the most sensitive one to the contaminations.
