E ects of arsenic on seed germination and physiological activities of wheatseedlings
LI Chun-xi , FENG Shu-li , SHAO Yun , JIANG Li-na , LU Xu-yang , HOU Xiao-li
DOI:
Received July 13, 2006,Revised December 01, 2006, Accepted , Available online
Volume 19,2007,Pages 725-732
- Summary
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The e ects of arsenic (As) were investigated on seed germination, root and shoot length and their biomass and some other factors
to elucidate the toxicity of As. The results showed low concentrations of As (0–1 mg/kg) stimulated seed germination and the
growth of root and shoot, however, these factors all decreased gradually at high concentrations of As (5–20 mg/kg). The contents
of O2
.??, MDA, soluble protein and peroxidase (POD) activity all increased with increasing As concentrations. Soluble sugar content,
ascorbate peroxidase (APX), and superoxide dismutase (SOD) activities decreased at low concentrations of As, and increased at high
concentrations of As. While acetylsalicylic acid (ASA) and chlorophyll contents, catalase (CAT) activity displayed increasing trend
when the concentrations of As was lower than 1 mg/kg, and then decreasing trend. By polyacrylamide gel electrophoresis (PAGE), As
induced the expression of POD isozymes of wheat seedlings. As induced the expression of CAT isozymes but inhibited the expression
of SOD isozymes of wheat seedlings at concentrations lower than 1 mg/kg. However, As inhibited the expression of CAT isozymes but
induced the expression of SOD isozymes at concentrations higher than 5 mg/kg. The results indicated As could exert harmfulness in
the early development stage of wheat at inappropriate concentrations.
