Environmental biology

Biodegradation of methyl parathion by Acinetobacter radioresistens USTB-04

LIU Fang-yao , HONG Ming-zhang , LIU Dan-mei , LI Ya-wen , SHOU Pei-shun , YAN Hai , SHI Guo-qing


Received October 09, 2006,Revised April 23, 2007, Accepted , Available online

Volume 19,2007,Pages 1257-1260

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Biodegradation of methyl parathion (MP), a widely used organophosphorus pesticide, was investigated using a newly isolated bacterium strain Acinetobacter radioresistens USTB-04. MP at an initial concentration of 1200 mg/L could be totally biodegraded by A. radioresistens USTB-04 as the sole carbon source less than 4 d in the presence of phosphate and urea as phosphorus and nitrogen sources, respectively. Biodegradation of MP was also achieved using cell-free extract of A. radioresistens USTB-04. MP at an initial concentration of 130 mg/L was completely biodegraded in 2 h in the presence of cell-free extract with a protein concentration of 148.0 mg/L, which was increased with the increase of pH from 5.0 to 8.0. Contrary to published reports, no intermediate or final degradation metabolites of MP could be observed. Thus we suggest that the cleavage of C–C bond on the benzene ring other than P–O bond may be the biodegradation pathway of MP by A. radioresistens USTB-04.

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