Alterations of organ histopathology and metallolhionein mRNA expression insilver barb, Puntius gonionotus during subchronic cadmium exposure
Alisa Wangsongsak , Suwimol Utarnpongsa , Maleeya Kruatrachue , Mathurose Ponglikitmongko , Prayad Pokethitiyook , Thitinun Sumranwanich
DOI:
Received February 13, 2007,Revised May 04, 2007, Accepted , Available online
Volume 19,2007,Pages 1341-1348
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Common silver barb, Puntius gonionotus, exposed to the nominal concentration of 0.06 mg/L Cd for 60 d, were assessed for
histopathological alterations (gills, liver and kidney), metal accumulation, and metallothionein (MT) mRNA expression. Fish exhibited
pathological symptoms such as hypertrophy and hyperplasia of primary and secondary gill lamellae, vacuolization in hepatocytes, and
prominent tubular and glomerular damage in the kidney. In addition, kidney accumulated the highest content of cadmium, more than
gills and liver. Expression of MT mRNA was increased in both liver and kidney of treated fish. Hepatic MT levels remained high after
fish were removed to Cd-free water. In contrast, MT expression in kidney was peaked after 28 d of treatment and drastically dropped
when fish were removed to Cd-free water. The high concentrations of Cd in hepatic tissues indicated an accumulation site or permanent
damage on this tissue.
