Environmental biology

Alterations of organ histopathology and metallolhionein mRNA expression insilver barb, Puntius gonionotus during subchronic cadmium exposure

Alisa Wangsongsak , Suwimol Utarnpongsa , Maleeya Kruatrachue , Mathurose Ponglikitmongko , Prayad Pokethitiyook , Thitinun Sumranwanich


Received February 13, 2007,Revised May 04, 2007, Accepted , Available online

Volume 19,2007,Pages 1341-1348

  • Summary
  • References
  • Related Articles
Common silver barb, Puntius gonionotus, exposed to the nominal concentration of 0.06 mg/L Cd for 60 d, were assessed for histopathological alterations (gills, liver and kidney), metal accumulation, and metallothionein (MT) mRNA expression. Fish exhibited pathological symptoms such as hypertrophy and hyperplasia of primary and secondary gill lamellae, vacuolization in hepatocytes, and prominent tubular and glomerular damage in the kidney. In addition, kidney accumulated the highest content of cadmium, more than gills and liver. Expression of MT mRNA was increased in both liver and kidney of treated fish. Hepatic MT levels remained high after fish were removed to Cd-free water. In contrast, MT expression in kidney was peaked after 28 d of treatment and drastically dropped when fish were removed to Cd-free water. The high concentrations of Cd in hepatic tissues indicated an accumulation site or permanent damage on this tissue.

Copyright © Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. and Science Press. All rights reserved.京ICP备05002858号-3