Effects of culture conditions on ligninolytic enzymes and protease productionby Phanerochaete chrysosporium in air
XIONG Xiaoping , WEN Xianghua , BAI Yanan , QIAN Yi
DOI:
Received March 21, 2007,Revised April 28, 2007, Accepted , Available online
Volume 20,2008,Pages 94-100
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The production of ligninolytic enzymes and protease by Phanerochaete chrysosporium was investigated under di erent culture
conditions. Di erent amounts of medium were employed in free and immobilized culture, together with two kinds of medium with
di erent C/N ratios. Little lignin peroxidase (LiP) (< 2 U/L) was detected in free culture with nitrogen-limited medium (C/N ratio:
56/2.2, in mmol/L), while manganese peroxidase (MnP) maximum activity was 231 and 240 U/L in 50 and 100 ml medium culture,
respectively. Immobilized culture with 50 ml nitrogen-limited medium gave the highest MnP and LiP production with the maximum
values of 410 and 721 U/L separately on the day 5; however, flasks containing 100 ml nitrogen-limited medium only produced less
MnP with a peak value of 290 U/L. Comparatively, carbon-limited medium (C/N ratio: 28/44, in mmol/L) was adopted in culture but
produced little MnP and LiP. Medium type had the greatest impact on protease production. Large amount of protease was produced due
to glucose limitation. Culture type and medium volume influence protease activity corporately by a ecting oxygen supply. The results
implied shallow immobilized culture was a possible way to gain high production of ligninolytic enzymes.
