DMPP (3,4-dimethylpyrazole phosphate) has been used to reduce nitrogen (N) loss from leaching or denitrification and to improve
N supply in agricultural land. However, its impact on soil nitrifying organisms and enzyme activities involved in N cycling is largely
unknown. Therefore, an on-farm experiment, for two years, has been conducted, to elucidate the e ects of DMPP on mineral N (NH4
+-
N and NO3??-N) leaching, nitrifying organisms, and denitrifying enzymes in a rice-oilseed rape cropping system. Three treatments
including urea alone (UA), urea + 1% DMPP (DP), and no fertilizer (CK), have been carried out. The results showed that DP enhanced
the mean NH4
+-N concentrations by 19.1%–24.3%, but reduced the mean NO3??-N concentrations by 44.9%–56.6% in the leachate,
under a two-year rice-rape rotation, compared to the UA treatment. The population of ammonia oxidizing bacteria, the activity of nitrate
reductase, and nitrite reductase in the DP treatment decreased about 24.5%–30.9%, 14.9%–43.5%, and 14.7%–31.6%, respectively, as
compared to the UA treatment. However, nitrite oxidizing bacteria and hydroxylamine reductase remained almost una ected by DMPP.
It is proposed that DMPP has the potential to either reduce NO3??-N leaching by inhibiting ammonia oxidization or N losses from
denitrification, which is in favor of the N conversations in the rice-oilseed rape cropping system.