Microbial detoxification of metalaxyl in aquatic system
Ahmed H. Massoud , Aly S. Derbalah , El-Sayed. B. Belal
DOI:
Received May 05, 2007,Revised August 23, 2007, Accepted , Available online
Volume 20,2008,Pages 262-267
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Four microorganisms, Pseudomonas sp. (ER2), Aspergillus niger (ER6), Cladosporium herbarum (ER4) and Penicilluim sp. (ER3),
were isolated from cucumber leaves previously treated with metalaxyl using enrichment technique. These isolates were evaluated for
detoxification of metalaxyl at the recommended dose level in aquatic system. The e ect of pH and temperature on the growth ability of
the tested isolates was also investigated by measuring the intracellular protein and mycelia dry weight for bacterial and fungal isolates,
respectively. Moreover, the toxicity of metalaxyl after 28 d of treatment with the tested isolates was evaluated to confirm the complete
removal of any toxic materials (metalaxyl and its metabolites). The results showed that the optimum degree pH for the growth of
metalaxyl degrading isolates (bacterial and fungal isolates) was 7. The temperature 30℃ appeared to be the optimum degree for the
growth of either fungal or bacterial isolates. The results showed that Pseudomonas sp. (ER2) was the most e ective isolate in metalaxyl
degradation followed by Aspergillus niger (ER6), Cladosporium herbarum (ER4) and Penicilluim sp. (ER3), respectively. There is
no toxicity of metalaxyl detected in the supernatant after 28 d of treatment with Pseudomonas sp. (ER2). The results suggest that
bioremediation by Pseudomonas sp. (ER2) isolate was considered to be e ective method for detoxification of metalaxyl in aqueous
media.
