Environmental biology

Effects of nicotinamide and riboflavin on the biodesulfurization activity ofdibenzothiophene by Rhodococcus erythropolis USTB-03

YAN Hai , SUN Xudong , XU Qianqian , MA Zhao , XIAO Chengbin , Ning Jun


Received July 08, 2007,Revised August 20, 2007, Accepted , Available online

Volume 20,2008,Pages 613-618

  • Summary
  • References
  • Related Articles
Rhodococcus erythropolis USTB-03 is a promising bacterial strain for the biodesulfurization of dibenzothiophene (DBT) via a sulfurspecific pathway in which DBT is converted to 2-hydroxybiphenyl (2HBP) as an end product. The effects of nicotinamide and riboflavin on the sulfur specific activity (SA) of DBT biodesulfurization by R. erythropolis USTB-03 were investigated. Both nicotinamide and riboflavin were found to enhance the expression of SA, which was not previously reported. When R. erythropolis USTB-03 was grown on a medium containing nicotinamide of 10.0 mmol or riboflavin of 50.0 mol, SA was raised from 68.0 or so to more than 130 mmol 2HBP/(kg dry cells h). When R. erythropolis USTB-03 was grown in the presence of both nicotinamide of 5.0 mmol and riboflavin of 25.0 mol, SA was further increased to 159.0 mmol 2HBP/(kg dry cells h). It is suggested that the biological synthesis of reduced form of flavin mononucleotide (FMNH2), an essential coenzyme for the activities of biodesulfurization enzyme Dsz C and A, might be enhanced by nicotinamide and riboflavin, which was responsible for the increased SA of R. erythropolis USTB-03.

Copyright © Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. and Science Press. All rights reserved.京ICP备05002858号-3