Molecular characterization of bacterial community in aerobic granular sludgestressed by pentachlorophenol
LIU He , LI Guangwei , LI Xiufen , CHEN Jian
DOI:
Received September 28, 2007,Revised November 28, 2007, Accepted , Available online
Volume 20,2008,Pages 1243-1249
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To characterize the e ects of pentachlorophenol (PCP) on the performance and microbial community of aerobic granular sludge in
sequencing batch reactor (SBR), the web-based terminal restriction fragment length polymorphism (T-RFLP) and real-time PCR (RTPCR)
techniques were used to explore the bacterial community structure. When PCP increased from 0 to 50 mg/L, the COD removal
rate changed little, while the ammonia removal rate dropped from 100% to 64.9%. The results of molecular characterization showed
that the quantity of ammonia oxidizing bacteria (AOB) kept constantly, although the number of bacteria species decreased with the
increase of PCP concentration. Significant shift in bacterial community structure at di erent PCP stresses was observed within aerobic
granular sludge. When the PCP was absent, there are 69 strains in aerobic granular sludge detected by T-RFLP method. With the
increase of PCP, most of bacteria disappeared and only 19 bacteria existed at all five PCP concentrations. These results contributed to
comprehensive understanding of the microbial community structure under the PCP stress and its relationship with the performance for
wastewater treatment by aerobic granular sludge.
