Degradation of o-chloronitrobenzene as the sole carbon and nitrogen sourcesby Pseudomonas putida OCNB-1
WU Haizhen , WEI Chaohai , WANG Yaqin , HE Qincong , LIANG Shizhong
DOI:
Received February 19, 2008,Revised April 12, 2008, Accepted , Available online
Volume 21,2009,Pages 89-95
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A bacterial strain that utilized o-chloronitrobenzene (o-CNB) as the sole carbon, nitrogen and energy sources was isolated from an
activated sludge collected from an industrial waste treatment plant. It was identified as Pseudomonas putida based on its morphology,
physiological, and biochemical characteristics with an automatic biometrical system and the 16S rRNA sequence analysis. Microcosm
study showed that the biodegradation of o-CNB was optimized at culture medium pH 8.0 and 32°C. At these conditions, the strain
degraded 85% of o-CNB at a starting concentration of 1.1 mmol/L in 42 h. o-Chloroaniline was identified as the major metabolite
with high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS). The study showed that
o-CNB degradation by Pseudomonas putida OCNB-1 was initiated by aniline dioxyenase, nitrobenzene reductase and catechol-1,2-
dioxygenase.
