Heavy metal induced DNA changes in aquatic macrophytes: Randomamplified polymorphic DNA analysis and identification of sequencecharacterized amplified region marker
Meetu Gupta , Neera Bhalla Sarin
DOI:
Received June 02, 2008,Revised July 18, 2008, Accepted , Available online
Volume 21,2009,Pages 686-690
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Plants have been used as good bio-indicators and genetic toxicity of environmental pollution in recent years. In this study, aquatic
plants Hydrilla verticillata and Ceratophyllum demersum treated with 10 mol/L Cd, 5 mol/L Hg, and 20 mol/L Cu for 96 h,
showed changes in chlorophyll, protein content, and in DNA profiles. The changes in DNA profiles included variation in band intensity,
presence or absence of certain bands and even appearance of new bands. Genomic template stability test performed for the qualitative
measurement of changes in randomly amplified polymorphic DNA (RAPD) profiles, showed significant e ect at the given concentration
of metals. Cloning and sequencing of bands suggested that these markers although may not be homologous to any known gene but its
conversion as a sequence characterized amplified region (SCAR) marker is useful in detecting the e ects of genotoxin agents.
