Isolation and characterization of Pseudomonas otitidis WL-13 and its capacityto decolorize triphenylmethane dyes
WU Jing , JUNG Byung-Gil , KIM Kyoung-Sook , LEE Young-Choon , SUNG Nak-Chang
DOI:
Received July 22, 2008,Revised November 16, 2008, Accepted , Available online
Volume 21,2009,Pages 960-964
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Pseudomonas otitidis WL-13, which has a high capacity to decolorize triphenylmethane dyes, was isolated from activated sludge
obtained from a wastewater treatment plant of a dyeing industry. This strain exhibited a remarkable color-removal capability when
tested against several triphenylmethane dyes under both shaking and static conditions at high concentrations of dyes. More than 95%
of Malachite Green and Brilliant Green was removed within 12 h at 500 mol/L dye concentration under shaking conditions. Crystal
Violet lost about 13% of its color under the same conditions tested. The rate of decolorization increased when the M9 medium was
supplemented with yeast extract. The optimum pH and temperature for color removal were 7–9 and 35–40°C, respectively. The observed
changes in the visible spectra and the inspection of bacterial growth indicated the color-removal by the adsorption of dye to the cells
during incubation with strains.
