Phylogenetic diversity and specificity of bacteria associated with Microcystisaeruginosa and other cyanobacteria
SHI Limei , CAI Yuanfeng , YANG Hualin , XING Peng , LI Pengfu , KONG Lingdong , KONG Fanxiang
DOI:
Received December 15, 2008,Revised March 03, 2009, Accepted , Available online
Volume 21,2009,Pages 1581-1590
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Interactions between bacteria and cyanobacteria have been suggested to have a potential to influence harmful algal bloom dynamics;
however, little information on these interactions has been reported. In this study, the bacterial communities associated with five strains of
Microcystis aeruginosa, three species of other Microcystis spp., and four representative species of non-Microcystis cyanobacteria were
compared. Bacterial 16S rDNA fragments were amplified and separated by denaturing gradient gel electrophoresis (DGGE) followed
by DNA sequence analysis. The similarities among bacterial communities associated with these cyanobacteria were compared to
the digitized DGGE profiles using the cluster analyses. The bacterial community structure of all cyanobacterial cultures di ered.
Cluster analysis showed that the similarity values among M. aeruginosa cultures were higher than those of other cyanobacterial
cultures. Sequence analysis of DGGE fragments indicated the presence of bacteria including, Alphaproteobacteria, Betaproteobacteria,
Gammaproteobacteria, Bacteroidetes and Actinobacteria in the cyanobacterial cultures. Members of the Sphingomonadales were the
prevalent group among the Microcystis-associated bacteria. The results provided further evidence for species-specific associations
between cyanoabcteria and heterotrophic bacteria, which are useful for understanding interactions between Microcystis and their
associated bacteria.
