Assessing the impact of fungicide enostroburin application on bacterialcommunity in wheat phyllosphere
Likun Gu , Zhihui Bai , Bo Jin , Qing Hu , Huili Wang , Guoqiang Zhuang , Hongxun Zhang
DOI:
Received April 10, 2009,Revised July 17, 2009, Accepted , Available online
Volume 22,2010,Pages 134-141
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Fungicides have been used extensively for controlling fungal pathogens of plants. However, little is known regarding the e ects that
fungicides upon the indigenous bacterial communities within the plant phyllosphere. The aims of this study were to assess the impact
of fungicide enostroburin upon bacterial communities in wheat phyllosphere. Culture-independent methodologies of 16S rDNA clone
library and 16S rDNA directed polymerase chain reaction with denaturing gradient gel electrophoresis (PCR-DGGE) were used for
monitoring the change of bacterial community. The 16S rDNA clone library and PCR-DGGE analysis both confirmed the microbial
community of wheat plant phyllosphere were predominantly of the
-Proteobacteria phyla. Results from PCR-DGGE analysis indicated
a significant change in bacterial community structure within the phyllosphere following fungicide enostroburin application. Bands
sequenced within control cultures were predominantly of Pseudomonas genus, but those bands sequenced in the treated samples were
predominantly strains of Pantoea genus and Pseudomonas genus. Of interest was the appearance of two DGGE bands following
fungicide treatment, one of which had sequence similarities (98%) to Pantoea sp. which might be a competitor of plant pathogens. This
study revealed the wheat phyllosphere bacterial community composition and a shift in the bacterial community following fungicide
enostroburin application.
