Characterization of phenanthrene degradation by strain Polyporus sp. S133
Tony Hadibarata , Sanro Tachibana
DOI:
Received March 09, 2009,Revised June 05, 2009, Accepted , Available online
Volume 22,2010,Pages 142-149
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Polyporus sp. S133, a fungus collected from contaminated soil, was used to degrade phenanthrene, a polycyclic aromatic
hydrocarbon, in a mineral salt broth liquid culture. A maximal degradation rate (92%) was obtained when Polyporus sp. S133
was cultured for 30 days with agitation at 120 r/min, as compared to 44% degradation in non-agitated cultures. Furthermore, the
degradation was a ected by the addition of surfactants. Tween 80 was the most suitable surfactant for the degradation of phenanthrene
by Polyporus sp. S133. The degradation rate increased as the amount of Tween 80 added increased. The rate in agitated cultures was
about 2 times that in non-agitated cultures. The mechanism of degradation was determined through the identification of metabolites;
9,10-phenanthrenequinone, 2,2’-diphenic acid, phthalic acid, and protocatechuic acid. Several enzymes (manganese peroxidase, lignin
peroxidase, laccase, 1,2-dioxygenase and 2,3-dioxygenase) produced by Polyporus sp. S133 were detected during the incubation. The
highest level of activity was shown by 1,2-dioxygenase (187.4 U/L) after 20 days of culture.
