Bacterial diversity in Fez tanneries and Morocco’s Binlamdoune River,using 16S RNA gene based fingerprinting
Adil Essahale , Moustafa Malki , Irma Mar′?n , Mohieddine Moumni
DOI:
Received September 08, 2009,Revised June 13, 2010, Accepted , Available online
Volume 22,2010,Pages 1944-1953
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Tannery wastewater causes serious ecological and sanitary damage. Chemical analysis of water from Binlamdoune River of the
medina of Fez was conducted and the results revealed the presence of toxic elements from tanneries and other industrial activities,
which strongly a ected water quality. To determine the e ectiveness of bioremediation for depollution, we studied the abundance
and diversity of bacteria residing in these polluted environments. Conducting denaturing gradient gel electrophoresis (PCR-DGGE)
of the 16S rDNA area using primers related to bacteria showed a bacterial community belonging to eubacterial groups, that is,
Epsilonproteobacteria, Clostridia, Lactobacillales, Bacteroidetes, Gammaproteobacteria, and Alphaproteobacteria. In addition, cloning
displayed the presence of clones belonging to the Firmicutes group. Moreover, scanning electron microscopy revealed a significant
heterogeneity of microorganism forms and structures. These endogenous microbes could have a significant role in the purification of
Binlamdoune River and Fez tannery wastewater.
