Enantioselective induction of oxidative stress by acetofenate in rat PC12 cells
Ling Li , Fen Hu , Cui Wang , Xuedong Wang
DOI:
Received December 24, 2009,Revised April 08, 2010, Accepted , Available online
Volume 22,2010,Pages 1980-1986
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In a non-chiral environment, the enantiomers of a racemate possessed the identical physico-chemical properties, but in the biological
systems they possessed di erent activities. Considering that the involvement of oxidative damage has been implicated in the toxicities of
various pesticides, this study investigated the possibility of enantioselective oxidative stress and cytotoxicity induction by acetofenate
(AF) which contains an asymmetrical center on PC12 cells. The results of the cytotoxicity assay indicated that S-(+)-AF presented
more toxic e ects than R-(–)-AF and ( )-AF. It also demonstrated that S-(+)-AF possessed the strongest e ects in induction of
reactive oxygen species (ROS) production, decrease in superoxide dismutase (SOD) and catalase (CAT) activities, and increase in
malondialdehyde (MDA) level. These results suggested that AF and its enantiomers could induce enantioselective cytotoxicity in PC12
cells mediated by oxidative stress. Therefore, the assessment in environmental safety and new chiral pesticide development should
consider enantioselectivity.
