Assessment of air pollution genotoxicity by RAPD in Evernia prunastri L. Ach.from around iron-steel factory in Karab¨uk, Turkey
Demet Cansaran-Duman , Orhan Atakol , S¨umer Aras
DOI:
Received June 14, 2010,Revised October 12, 2010, Accepted , Available online
Volume 23,2011,Pages 1171-1178
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Lichens are widely used in biomonitoring studies of air pollution, either as bioindicators of air quality or as bioaccumulators
of atmospheric deposition. Over the past decade, several molecular techniques have been developed to provide information on
diversity, genotoxicology, genetic relationships, etc. The heavy metal contents of Evernia prunastri samples were determined by
atomic absorption spectrometry. The Random Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR) method was
used to describe the pattern of DNA band variation in the samples influenced by the environmental pollution. The study was designed
to describe the level of pollution in an area contaminated with smoke and waste from an iron-steel factory, and to reveal the level
of potential genotoxic agents around this source of pollution. The study also examined the suitability of the lichen samples for the
detection of genotoxicity.
