Cadmium accumulation and tolerance of two castor cultivars in relation to antioxidant systems

Hanzhi Zhang , Qingjun Guo , Junxing Yang , Tongbin Chen , Guangxu Zhu , Marc Peters , Rongfei Wei , Liyan Tian , Chunyu Wang , Deyun Tan , Jie Ma , Gangming Wang , Yingxin Wan


Received November 06, 2013,Revised February 19, 2014, Accepted , Available online October 22, 2014

Volume 26,2014,Pages 2048-2055

To investigate the effects of Cd on tolerance and antioxidant activities of castor, two different castor (Ricinus communis L.) cultivars (Zibo No. 5 and Zibo No. 8) were used for a hydroponic experiment (0, 1 and 2 mg/L Cd) and a pot experiment using Cd-contaminated soil (34 mg/kg) with the addition of ethylenedinitrilotetraacetic acid (EDTA). The results indicated that there were significant differences between the two cultivars with respect to Cd uptake in shoots (113-248 mg/kg for Zibo No. 5 and 130-288 mg/kg Zibo No. 8), biomass tolerance indexes (64.9%-74.6% for Zibo No. 5 and 80.1%-90.9% for Zibo No. 8) in the hydroponic experiment and survival rates (0% for Zibo No. 5 and 100% for Zibo No. 8) determined by the addition of EDTA in the pot experiment, suggesting that Zibo No. 8 has higher tolerance than Zibo No. 5. Moreover, the castor cultivars have low bioconcentration factors (4.80% for Zibo No. 5 and 5.43% for Zibo No. 8) and low translocation factors (<1%). Consequently, Zibo No. 8 can participate in Cd phytostabilization in highly Cd-polluted areas. The results indicated that glutathione (GSH) as a non-enzymatic antioxidant, and antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT) and guaiacol peroxidase (GPX), were cultivar- and dose-dependent. The higher tolerance of Zibo No. 8 compared with Zibo No. 5 can be attributed to the higher GSH levels in the root and higher GPX activity in the leaf.

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