Molecular characterization of microbial communities in bioaerosols of a coal mine by 454 pyrosequencing and real-time PCR

Min Wei , Zhisheng Yu , Hongxun Zhang


Received May 19, 2014,Revised June 25, 2014, Accepted , Available online April 23, 2015

Volume 27,2015,Pages 241-251

Microbial diversity and abundance in bioaerosols of a coal mine were analyzed based on 454 pyrosequencing and real-time polymerase chain reaction (PCR). A total of 37,191 high quality sequenceswere obtained and could be classified into 531, 1730 and 448 operational taxonomic units respectively for archaea, bacteria and fungi at 97% sequence similarity. The Shannon diversity index for archaea, bacteria and fungiwas respectively 4.71, 6.29 and 3.86, indicating a high diversity in coal mine bioaerosols. Crenarchaeota, Proteobacteria and Ascomycota were the dominant phyla for archaea, bacteria and fungi, respectively. The concentrations of total archaea, bacteria and fungi were 1.44 × 108, 1.02 × 108 and 9.60 × 104 cells/m3, respectively. Methanotrophs observed in bioaerosols suggested possible methane oxidation in the coal mine. The identified potential pathogens to coal miners, such as Acinetobacter schindleri, Aeromonas cavernicola, Alternaria alternata, Aspergillus penicillioides, Cladosporium cladosporioides, and Penicilliumbrevicompactumwere also observed. Thiswas the first investigation ofmicrobial diversity and abundance in coalmine bioaerosols. The investigation ofmicrobial communities would be favorable in promoting the progress of methane control based on microbial technique and concern on coal miners' health.

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