Differences in nitrite-oxidizing communities and kinetics in a brackish environment after enrichment at low and high nitrite concentrations
Wipasanee Tangkitjawisut , Tawan Limpiyakorn , Sorawit Powtongsook , Preeyaporn Pornkulwat , Benjaporn Boonchayaanant Suwannasilp
DOI:10.1016/j.jes.2015.07.014
Received March 17, 2015,Revised July 17, 2015, Accepted August 05, 2015, Available online October 29, 2015
Volume 28,2016,Pages 41-49
Nitrite accumulation in shrimp ponds can pose serious adverse effects to shrimp production and the environment. This study aims to develop an effective process for the enrichment of ready-to-use nitrite-oxidizing bacteria (NOB) inocula that would be appropriate for nitrite removal in brackish shrimp ponds. To achieve this objective, the effects of nitrite concentrations on NOB communities and nitrite oxidation kinetics in a brackish environment were investigated. Moving-bed biofilm sequencing batch reactors and continuous moving-bed biofilm reactors were used for the enrichment of NOB at various nitrite concentrations, using sediment from brackish shrimp ponds as seed inoculum. The results from NOB population analysis with quantitative polymerase chain reaction (qPCR) show that only Nitrospira were detected in the sediment from the shrimp ponds. After the enrichment, both Nitrospira and Nitrobacter coexisted in the reactors controlling effluent nitrite at 0.1 and 0.5 mg-NO2−-N/L. On the other hand, in the reactors controlling effluent nitrite at 3, 20, and 100 mg-NO2−-N/L, Nitrobacter outcompeted Nitrospira in many orders of magnitude. The half saturation coefficients (Ks) for nitrite oxidation of the enrichments at low nitrite concentrations (0.1 and 0.5 mg-NO2−-N/L) were in the range of 0.71–0.98 mg-NO2−-N/L. In contrast, the Ks values of NOB enriched at high nitrite concentrations (3, 20, and 100 mg-NO2−-N/L) were much higher (8.36–12.20 mg-NO2−-N/L). The results suggest that the selection of nitrite concentrations for the enrichment of NOB inocula can significantly influence NOB populations and kinetics, which could affect the effectiveness of their applications in brackish shrimp ponds.
