Robustness of anammox granular sludge treating low-strength sewage under various shock loadings: Microbial mechanism and little N2O emission
Shanyun Wang , Guibing Zhu , Yifei Li , Xiaoxia Wang , Jiemin Zhou , Yongzhen Peng
DOI:10.1016/j.jes.2019.03.016
Received January 15, 2019,Revised , Accepted March 18, 2019, Available online May 31, 2019
Volume 31,2019,Pages 141-153
With the increasing application of anammox for the treatment of high-strength industrial wastewater, application of anammox in municipal sewage has been gaining more attention. Sludge granulation in particular enhances the enrichment and retention of anammox bacteria in municipal sewage treatment systems. However, the performance of granular sludge under continuous and varying hydraulic loading shock remains little understood. In this study, the robustness of anammox granular sludge in treating low-strength municipal sewage under various shock loadings was investigated. Results showed that an upflow anaerobic sludge blanket (UASB) reactor with anammox granules performed well, with anammox specific activity up to 0.28 kg N/kg VSS/day and anti-loading shock capability up to 187.2 L/day during the 8-month testing period. The accumulation rate of N2O (< 0.01 kg N/kg VSS/day) in the liquid phase was seven times higher than that of the gas phase, which could be mainly attributed to the incomplete denitrification and insufficient carbon source. However, only a small part of the produced N2O escaped into the atmosphere. High-throughput sequencing and molecular ecological network analyses also identified the bacterial diversity and community structure, indicating the potential resistance against loading shock. The composition and structural analyses showed that polysaccharides were an important functional component in the tightly bound extracellular polymeric substances (TB-EPS), which was the major EPS layer of anammox granules. Scanning electron microscopy (SEM) also showed that the gaps in between the anammox-clusters in the granules inhibit the flotation of the sludge and ensure efficient settling and retention of anammox granules.
