Effects of different carbon sources on the removal of ciprofloxacin and pollutants by activated sludge: Mechanism and biodegradation

Jiachao Zhang , Yixiang Cai , Zhiyong Yan , Yingjuan Ou , Boshang Peng , Lihua Zhang , Jihai Shao , Yiqing Lin


Received December 11, 2020,Revised , Accepted March 23, 2021, Available online April 11, 2021

Volume 34,2022,Pages 240-248

This research investigated the effects of ciprofloxacin (CIP) (0.5, 5, and 20 mg/L) on SBR systems under different carbon source conditions. Microbial community abundance and structure were determined by quantitative PCR and high-throughput sequencing, respectively. The biodegradation production of CIP and possible degradation mechanism were also studied. Results showed that CIP had adverse effects on the nutrient removal from wastewater. Compared with sodium acetate, glucose could be more effectively used by microorganisms, thus eliminating the negative effects of CIP. Glucose stimulated the microbial abundance and increased the removal rate of CIP by 18%–24%. The mechanism research indicated that Proteobacteria and Acidobacteria had a high tolerance for CIP. With sodium acetate as a carbon source, the abundance of nitrite-oxidizing bacterial communities decreased under CIP, resulting in the accumulation of nitrite and nitrate. Rhodanobacter and Microbacterium played a major role in nitrification and denitrification when using sodium acetate and glucose as carbon sources. Dyella and Microbacterium played positive roles in the degradation process of CIP and eliminated the negative effect of CIP on SBR, which was consistent with the improved removal efficiency of pollutants.

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