Spatial distribution of bacterial resistance towards antibiotics of rural sanitation system in China and its potential link with diseases incidence
Yan Xu , Houyu Li , Lu Tan , Chunxue Zhang , Xiaocheng Wei , Qiang Wang , Qian Li , Xiangqun Zheng
DOI:10.1016/j.jes.2022.06.010
Received May 09, 2022,Revised , Accepted June 06, 2022, Available online June 24, 2022
Volume 35,2023,Pages 361-374
Chinese government is vigorously promoting toilet renovation in rural areas to reduce the risk of human feces exposure, which would cause infectious diseases, especially antibiotic resistance genes (ARGs) and pathogens. However, the distribution of ARGs in human feces from different regions of China remained ill-defined. It is not yet known how the survival of ARGs after toilet treatment is associated with the regional infection rates. Here, we investigated the prevalence of ARGs in human feces in rural areas of China and their potential relationship with infectious diseases for the first large-scale. The results showed that there were still high ARGs residues in human feces after rural toilet treatment, especially tetM-01 and ermB with average relative abundance as high as 1.21 × 10−1 (Eastern) and 1.56 × 10−1 (Northern), respectively. At a large regional scale, the significant differences in human feces resistomes were mainly shaped by the toilet types, TN, NH3-N, and the bacterial community. A critical finding was that toilets still cannot effectively decrease the pathogenicity risk in human feces. The significant positive relationship (P<0.05) between infectious diseases and ARGs can infer that ARGs in human feces exposure might be a critical path for enhancing the incidence of diseases, as these ARGs hinder the effectiveness of antibiotics.
